O estudo exploratório, de abordagem qualitativa, objetivou investigar o relacionamento intergeracional e as práticas de apoio presentes no cotidiano de famílias de crianças com necessidades especiais. Participaram do estudo cinco mães, avós e irmãos mais velhos de crianças com deficiência, que responderam a roteiros de entrevistas semiestruturadas. As entrevistas foram analisadas qualitativamente por meio da técnica do Discurso do Sujeito Coletivo. Verificou-se que as avós apresentam-se como importante fonte de apoio à família ao dedicarem, inclusive, atenção e cuidado ao irmão da criança com necessidades especiais. O estudo revela um relacionamento intergeracional positivo entre avós e netos. Destaca-se a necessidade de direcionar maior atenção especializada a esses membros da família e para a continuidade de investigação sobre o tema.
SUMMARYStrongyloidiasis is a potentially serious infection in immunocompromised patients.
Thus, the availability of sensitive and specific diagnostic methods is desirable,
especially in the context of immunosuppressed patients in whom the diagnosis and
treatment of strongyloidiasis is of utmost importance. In this study, serological and
molecular tools were used to diagnose Strongyloides stercoralis
infections in immunosuppressed patients. Serum and stool samples were obtained from
52 patients. Stool samples were first analyzed by Lutz, Rugai, and Agar plate culture
methods, and then by a quantitative real time polymerase chain reaction (qPCR). Serum
samples were evaluated by an enzyme-linked immunosorbent assay (ELISA) using a
soluble (AS) or a membrane fractions antigen (AM) obtained from alkaline solutions of
the filariform larvae of Strongyloides venezuelensis. Of the 52
immunosuppressed patients, three (5.8%) were positive for S.
stercoralis by parasitological methods, compared to two patients (3.8%)
and one patient (1.9%) who were detected by ELISA using the AS and the AM antigens,
respectively. S. stercoralis DNA was amplified in seven (13.5%)
stool samples by qPCR. These results suggest the utility of qPCR as an alternative
diagnostic tool for the diagnosis of S. stercoralis infection in
immunocompromised patients, considering the possible severity of this helminthiasis
in this group of patients.
Editor Científico: Tonny Kerley de Alencar Rodrigues Artigo recebido em 04/08/2017. Última versão recebida em 18/09/2017. Aprovado em 19/09/2017. Avaliado pelo sistema Triple Review: a) Desk Review pelo Editor-Chefe; e b) Double Blind Review (avaliação cega por dois avaliadores da área).
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