Objective: The present research was conducted to identify the prevalence of Anisakis sp. as fish-borne zoonoses in Epinephelus sp. in territorial waters of East Nusa Tenggara, Indonesia. Materials and methods: A total of 50 fish (Epinephelus sp.) were collected from Kupang Fish Market in East Nusa Tenggara. Identification of Anisakis sp. was performed based on morphological observations considering shape of ventriculus, boring tooth, and mucron using binocular microscope. Results: Prevalence of Anisakis sp. in Epinephelus sp. was 22% (n=11/50). The parasite was mostly found in abdominal cavity, gonad, intestines, and muscles of fish. Conclusion: This study suggests that Anisakis sp. is present in Epinephelus sp., exerting potential health hazards for human who consume the fish.

The Culex mosquito is a species that can creates health problems for humans and animals. The handling efforts of the vector is very important to reduce the impact caused by this vector. One of the plants that has the potential as a larvicide is soursop leaves (Annona muricata L.). This study aims to determine the effect of soursop leaves (Annona muricata L.) extract on the mortality of Culex sp larvae and LC50 value to killing 50% Culex sp larva. The study was conducted from March to June 2020. This study used 7 treatment groups which 5 groups tested the effectiveness of soursop leaves extract and 2 control groups. The research data was analyzed using the Probit test to determine the LC50. The results showed that soursop leaves extract (Annona muricata L.) was effective in killing Culex sp larvae with LC50 value is 0.736%.

Pengendalian nyamuk yang berperan sebagai vektor  merupakan tindakan terpenting dalam penanggulangan penyakit seperti filariasis.Filariasis merupakan penyakit menular yang disebabkan oleh cacing filaria yang menyerang saluran dan kelen­jar getah bening yang ditularkan oleh berba­gai jenis nyamuk. Kasus filariasis di Kabupaten Sikka tahun 2015- 2017 setiap tahunnya meningkat. Penggunaan insektisida menjadi pilihan utama masyarakat dalam pengendalian vektor nyamuk. Insektisida yang digunakan secara terus-menerus dapat menyebabkan nyamuk menjadi resisten. Penelitian ini bertujuan untuk mengetahui status resistensi vektor filariasis terhadap insektisida permethrin 0,75%  di Kabupaten Sikka tahun 2018. Penelitian ini diawali dengan survei lokasi dan pengambilan sampel di Kabupaten Sikka dan dilanjutkan dengan pemeliharaan nyamuk serta uji resistensi. Bahan yang digunakan dalam penelitian ini adalah nyamuk Aedes sp. dan Anopheles sp., impregnated paper permethrin 0,75%.  Hasil uji resistensi menggunakan metode susceptibility test dengan impregnated paper permethrin 0,75% adalah kematian nyamuk uji terhadap permethrin adalah 21,3% sehingga disimpulkan bahwa vektor filariasis di Kabupaten Sikka dinyatakan telah resisten tinggi terhadap insektisida permethrin 0,75% karena kematian nyamuk uji <90%.

Dengue hemorrhagic fever (DHF) is a disease that is found in some tropical and subtropical regions. This disease is caused by dengue virus and is transmitted to humans through the bite of the Aedes aegypti mosquito. the solution taken in controlling DHF is to break the life cycle of the Aedes aegypti mosquito. Vector control is generally carried out using synthetic larvicides, namely abate / temefos, but the use of abate can cause residues, environmental pollution, poisoning and resistance of the eradicated vectors so that natural larvasides from plants are needed namely temulawak rhizome (Curcuma xanthorrhiza Roxb.) for vector control. The purpose of this study was to determine whether temulawak rhizome extract was effective or not in killing Aedes aegypti larvae. This research method includes larva collection, identification and maintenance of mosquitoes, determining sample size, making extracts and testing effectiveness. This study used a control and experiment group with 3 repetitions in the minutes to 15, 30, 45, 60 and 1440 (24 Hours). The control group was positive control using abate and negative control using aquades while the eksperiment group used extract of temulawak rhizome (Curcuma xanthorrhiza Roxb.) with concentrations of 0.6%, 0.8%, 1%, 1.2% and 1.5%. The results of this study indicate that the temulawak rhizome extract (Curcuma xanthorrhiza Roxb.) effective as larvicides because at the lowest concentration 0.6% can kill 100% Aedes aegypti larvae.

Brown tick, Rhicephalus sanguineus generally known as a very adaptive tick, is widely spread on tropical, subtropical, and temperate regions, and infested dogs living on not only rural but urban areas. Synthetic acaricides are the most widely control measures. However, with the development of tick resistance, plant-derived components are highly investigated. The present study aims to investigate the activity of Crecentia cujete Linn. leaves extract on R. sanguineus. Fifty adult R. sanguineus were collected from local dogs and used in this study. Ticks are divided into the control and treatment groups. Aquadest and deltamethrin were sprayed to ticks in the negative and positive control groups, respectively. The three treatment groups were given maja leaves extract of 2.5%, 5%, and 10%. The tick mortality was observed for 12 hours of exposure to the extracts. All extracts exhibited similar lethal effects on R. sanguineus with total mortality (100%) after 4 hours exposure, compared to the standard therapy. The highest extract concentration (10%) reduces ticks faster compare to other concentrations. Thus, this study suggests that strong acaricidal activity and mortality rate was dose-dependent. This finding needs further scientific investigation to prove.