The aqueous-core enclosed in lipid nanoballoons integrating multiple emulsions of the type water-in-oil-in-water mimic, at least in theory, the environment within viable cells, thus being suitable for housing hydrophilic protein entities such as bioactive proteins, peptides and bacteriophage particles. This study reports a complete physicochemical characterization of optimized biomimetic aqueous-core lipid nanoballoons housing hydrophilic (BSA) protein entities, evolved from a statistical 2×3 factorial design study (three variables at two levels and one variable at three levels) that was the subject of the first paper of a series of three, aiming at complete stabilization of the three-dimensional structure of protein entities attempted via housing the said molecular entities within biomimetic aqueous-core lipid nanoballoons integrating a multiple (W/O/W) emulsion. The statistical factorial design followed led to the production of an optimum W/O/W multiple emulsion possessing quite homogeneous particles with an average hydrodynamic size of (186.2 ± 2.6) nm and average Zeta potential of (-36.5 ± 0.9) mV, and exhibiting a polydispersity index of 0.206 ± 0.014. Additionally, the results obtained for the diffusion coefficient of the lipid nanoballoons integrating the optimized W/O/W multiple emulsion were comparable and of the same order of magnitude (10 m s) as those published by other authors since, typically, diffusion coefficients for molecules range from 10 to 10 m s, but diffusion coefficients for nanoparticles are typically of the order of magnitude of 10 m s.

Este artigo pretende examinar os sepultamentos realizados no cemitério dos Pretos Novos, à luz da abordagem da história cultural. Buscando analisar os aparelhos simbólicos partilhados por ambas as culturas, a fim de resgatar a especificidade do referido campo santo e o seu lugar na sociedade brasileira dos séculos XVII a XIX.

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