International audienceA vision system is used for measuring in-plane target position, displacement and orientation. An encrypted pseudo-periodic pattern fixed on the target forms a phase reference. Absolute position is determined with subpixel accuracy by phase computations from any local view of a small zone of the pattern. Method principle is presented and performances are characterized. The capability to resolve position on depth ranges larger than the lens depth of focus is demonstrated. The method is applied to position referencing of live cell culture boxes and could be used as visual control sensor in other applications

International audienceThis work presents micro-grids integrated to cell culture boxes. These grids allow the systematic registration of the position of a zone observed by optical microscopy in a such way that it is possible to find it again easily for new observations for instance after culture on drug injection. The position knowledge allows also the numerical superimposition of recorded images in a common position reference system with a sub-pixel precision. It become thus straightforward to perform a site by site analysis of the possible evolutions that may have occurred in the biological medium

This work presents micro-grids integrated to cell culture boxes. These grids allow the systematic registration of the position of a zone observed by optical microscopy in a such way that it is possible to find it again easily for new observations for instance after culture on drug injection. The position knowledge allows also the numerical superimposition of recorded images in a common position reference system with a sub-pixel precision. It become thus straightforward to perform a site by site analysis of the possible evolutions that may have occurred in the biological medium.