The Breast Benign tumor (BBT) is currently considered a significant breast health problem within women of all ages. In the present study, we aimed to investigate the Tumor markers CA125 and CA153, thyroid hormones free triiodothyronine (FT3), free thyroxine (FT4), thyroid-stimulating hormone (TSH), and vitamin D within benign breast tumor (BBT) during the reproduction ages. Twenty patients (ten patients in the control group and ten patients in cases (Breast Benign Tumor); Notably, the studies confirm the decrease in FT4 and FT3 and increase of the thyroid-stimulating hormone (TSH). In addition, the decreased level of Vitamin D is also revealed in BBT.