Changes in nutrient availability trigger massive rearrangements of the yeast plasma membrane proteome. This work shows that the arrestin-related protein Csr2/Art8 is regulated by glucose signaling at multiple levels, allowing control of hexose transporter ubiquitylation and endocytosis upon glucose depletion.

Bul1 is identified as a novel actor in glucose-induced endocytosis, mainly acting at the plasma membrane in the process of Jen1 internalization. Bul1 acts as an alternate adaptor for another glucose-regulated ART protein, Rod1, to allow robustness in the process of glucose-induced endocytosis.