Introduction: By observing the characteristics of the development of competitive tennis in the world, it is not difficult to find that tennis requires extremely fast reaction times from players. Objective: To explore the relationship between athlete's reaction time and different training methods. Methods: 48 outstanding female tennis players were selected. They received four weeks of regular training (from March 2 to March 28, 2020) and two weeks before the competition (from June 8 to June 20, 2020). After the training, the Omega Wave system was used for testing, and urine samples were taken for catecholamine testing. Results: In the test of 48 people before the competition, 30 people experienced central fatigue, from which 24 people (80%) were in the regular training stage, and only six people (20%) were in the training stage before the competition. HRV indicators: In the regular training stage, the results of time-domain indicators such as SDNN (NN interval standard deviation), SDSD (NN interval difference standard deviation), RMSSD (NN interval difference root mean square), and frequency-domain indicators such as LF (low frequency) and HF (high frequency) in the second and third week of the athletes were lower than those in the first and fourth week, showing the characteristics of first falling and then rising. However, there was no significant change. Conclusion: Different training methods can improve athletes› competitive level and reaction speed. Level of evidence II; Therapeutic studies - investigation of treatment results.