The cDNA coding rabbit glutathione peroxidase was isolated from liver cDNA library in lambda gtll by cross hybridization with the rat glutathione peroxidase cDNA which was cloned in this laboratory and reported elsewhere(l). The cDNA consisted of 600 bp of the coding region and the nucleotide sequence revealed that TGA, which is to be the stop codon in general, encoded seleno-cysteine(SeC) residue as was proved to be so with glutathione peroxidases of mouse (2), man(3) and rat(l). The amino acid sequence deduced from cDNA possesses 84, 84, 87 and 85% homology with rat, mouse, human, and bovine(4) enzymes, respectively.