This study aims to determine the effect of cooperative culture, discipline culture, and classroom management skills on the achievement of strengthening 21st-century literacy in Senior High School (SMA/K) PE learning in Southeast Sulawesi Province. This research is a type of correlational research with a quantitative approach to determine the relationship between two or more variables. Data collection techniques through questionnaires. The sample of this study was 40 respondents of PE teachers SMA/K who were taken using the purposive sampling technique. The instruments in this study have been tested for validity and reliability, and have been consulted by experts. The data analysis technique uses regression analysis that has been tested for classical assumptions. Based on the results (T-test), the significant value of the cooperative culture variable is 0.133 > 0.05, discipline culture is 0.048 < 0.05 and class management skills is 0.000 < 0.05. This means that partially the X1 variable has no effect on Y, while the X2 and X3 variables have an effect on the Y variable. The ANOVA (F test) results obtained a significant value of 0.000. This shows that the culture of cooperation, discipline culture, and classroom management skills simultaneously affect the achievement of strengthening 21st-century literacy in SMA/K PE learning in the Southeast Sulawesi Province. In addition, the R square ® value of 0.854 means that 85.4% of the achievement of strengthening 21st-century literacy in PE SMA/K is influenced by a culture of cooperation, discipline culture, and classroom management skills.