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Inorganic substances play a significant role in the physiology of parasites and their importance, especially to gastro-intestinal parasites, is also demonstrated by the influence of mineral deficiencies in the host's diet (see Von Brand, 1966).

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Summary:Whole adult soluble extract of Haemonchus contortus as an antigen along with Freund's complete adjuvant, was used to immunize rabbits. Antisera from immunized rabbits were collected at intervals of 30, 60, 90, 1 20, 1 50 and 1 80 days. For the detection and titration of anti-H. contortus antibodies in these sera, Dot-ELISA was developed. Sera collected 30 days postimmunization exhibited a litre of 1:5,000 in all the rabbits except one, where a titre of 20,000 was recorded. Later, all the rabbits attained the highest titre of 40,000 at different periods of postimmunizations, which were maintained 150-180 days. These high titre sera can be of immense use in the identification and characterization of immunodominant antigens of adult H. contortus. KEY WORDS :Haemonchus contortus, Dot-ELISA, antigen, immune response. Résumé : ÉTUDE PAR DOT-ELISA DE LA RÉPONSE IMMUNITAIRE DE LAPINS IMMUNISÉS PAR DES ANTIGÈNES D'HAEMONCHUS CONTORTUS