In all organisms the only known ab initio pathway leading to synthesis of myoinositol is conversion of D-glucose-6-phosphate to L-myo-inositol-1-phosphate (via inositol-1-phosphate synthase, IPS), followed by specific dephosphorylation via inositol monophosphatase (IMPase). We have investigated both enzymes involved in synthesis of myo-inositol in the hyperthermophilic sulfate reducer Archaeoglobus fulgidus. The structures of both enzymes were obtained to better than 2 Å resolution. The structure solution process and the resulting structures of both enzymes will be described in detail and compared with corresponding enzymes from other species. Insights into catalysis and possible evolutionary changes will be discussed. In particular, we will focus on the use of metal ions in the IPS catalytic cycle and the basis for dual activity of the IMPase.

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