The south of Western Siberia traditionally belongs to the regions with an unfavorable epidemiological situation for infections transmitted by ticks. Approximately 20 thousand people annually seek medical care after a tick bite in Novosibirsk and its suburbs. The purpose of this work was to determine the species composition of ticks attacking humans in the territory of Novosibirsk and its suburbs by determining the nucleotide sequence of the fragment of the tick mitochondrial gene cytochrome oxidase COI during the spring-summer season of 2018, determination of infection of individual ticks with viral and bacterial tick-borne infections, followed by genotyping of identified pathogens by PCR methods, by determination of nucleotide sequences and phylogenetic analysis. It is established that ticks of five species attack the humans: Ixodes persulcatus, Ixodes pavlovskyi, Dermacentor reticulatus, Dermacentor marginatus, Dermacentor nuttali in the Novosibirsk metropolis. The majority of tick attacks on humans are associated with I. pavlovskyi (43.6%) and D. reticulatus (41.2%) ticks. The genetic material of the tick-borne encephalitis virus was detected in 3.6%, borrelia - 13.8% and rickettsia - 23.1% individual ticks collected from humans. Genotyping of the tick-borne encephalitis virus (TBEV) in the collected ticks showed the presence of the Siberian genotype of TBEV in 83% cases, and the Far Eastern genotype of TBEV in 17% of ticks. The Kemerovo virus could not be detected. Genetic material from Borrelia spp. and Rickettsia spp. has been detected in all five tick species attacking the local human population. The bulk of the identified Borrelia spp. isolates were genotyped as Borrelia garinii (86%) and 13% of the isolates as Borrelia afzelii species. The genetic material of Borrelia miyamotoi was found in one individual tick. Ticks of the genus Dermacentor have been found to infect by Rickettsia raoultii, and ticks of the genus Ixodes are overwhelmingly infected with Rickettsia tarasevichiae. Moreover, in single I. persulcatus tick was infected by Rickettsia helvetica. Thus, the human population of Novosibirsk and its suburbs is attacking by five species of ixodid ticks with the predominant dominance of I. pavlovskyi and D. reticulatus ticks. The genetic material of the following tick-borne infections was found: Siberian and Far Eastern genotype of TBEV, B. garinii, B. afzelii, B. miyamotoi, R. raoulti, R. tarasevichiae and R. helvetica in the individual ticks collected from humans.