Background: Joint health is very vital in canines. Immediate attention and diagnosis will help to prevent suffering in canines. This study was designed to evaluate the in vivo efficacy of the poly-herbal formulation Mobility Plus® for arthritis, inflammation and analgesic activities in canines. Methods: A total of 18 client-owned dogs with a history of joint inflammation, hip dysplasia and arthritis were selected (n = 18) and supplemented with one tablet of Mobility Plus® daily until complete recovery. The changes in assessment parameters scores, viz. lameness score, joint mobility score, pain on palpation score, weight-bearing score and the overall clinical condition score were evaluated. The results revealed that lameness, joint mobility, pain and weight-bearing scores significantly (p less than 0.001) decreased in dogs as early as day 15; hence, the overall clinical condition score also decreased significantly (p less than 0.001) as early as day 15 after Mobility Plus® supplementation. Result: Present study revealed that lameness, joint mobility, pain and weight-bearing scores significantly (p and lt; 0.001) decreased in dogs as early as day 15; hence, the overall clinical condition score also decreased significantly (p and lt; 0.001) as early as day 15 after Mobility Plus® supplementation. Supplementation of Mobility Plus® has antiarthritic and anti-inflammatory activities. Mobility Plus® could be recommended for the amelioration of joint inflammation and osteoarthritis conditions in canines.