Two simple, sensitive, accurate, rapid, and economical spectrophotometric methods have been developed for the estimation of diacerein in Pharmaceutical dosage forms. Method A is based on the reaction of diacerein with Folin-Ciocalteu reagent, in the presence of 0.5 N sodium hydroxide solution, giving a pink-colored chromogen, which shows maximum absorbance at 512 nm against reagent blank, while method B is based on the oxidation of diacerein with potassium permanganate in an alkaline medium giving a pink-colored chromogen, which shows maximum absorption at 497.5 nm. Beer’s law was obeyed in the concentration range of 4 – 20 µg/ml for both methods A and B. Results of the analysis were validated statistically, and by recovery studies.

Objective: The present work was done to find out various bioactive compounds present in the chloroform extract of Lycoperdon pyriforme using gas chromatography-mass spectrometry (GC-MS). Methods: L. pyriforme was collected cleaned to remove any dirt and oven dried at 60°C. The sample was then finely powdered and extracted with chloroform using Soxhlet apparatus. The chemical composition of chloroform extract was then analyzed using QP2010S-Shimadzu GC-MS instrument. Results: Chloroform extract resulted in the presence of 33 compounds with 1-Heneicosanol (11.17%) and E-15-Heptadecenal (11.08%) forming major compounds and 1-Tetradecanol (0.16%), Dichloroacetic acid, and decyl ester (0.15%) forming least. Conclusion: The results indicated the presence of a variety of compounds thus providing the information about various bioactive compounds present in Lycoperdon sp. and its further application in the field of pharmacology.