A field experiment was conducted during the rabi season of 2019-2020 at research farm, J.N.K.V.V., College of Agriculture, Tikamgarh (Madhya Pradesh) to study the “Effect of different levels of sulphur on growth and yield of onion (Allium cepa L.)”. The treatments consisted of four levels of sulphur, viz.( S0 - 0kg S/ha, S1 - 20 kg S/ha, S2 - 40 kg S/ha and S3 - 60 kg S/ha) were laid out in Randomized Block Design with three replications. Result showed significant influence on growth and yield attribute for different sulphur levels. Maximum plant height (62.62 cm), number of leaves per plant (10.25), leaf length (55.45 cm), leaf diameter(0.95 cm), neck thickness (1.88 cm), bulb length (5.73 cm), polar diameter (5.99cm), equatorial diameter (6.11 cm), bulb weight (91.32 g) bulb yield (20.30 kg/plot) and bulb yield (282.00 q/ha) were recorded with application of sulphur 60 kg/ha. Which justify its superiority over rest of the treatment while minimum Growth and Yield parameters were recorded 0kg S/ha.