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The influence of kinetin and sucrose on (60%), Hill activity (about 3-fold), and "C-fixation from NaH"CO3 (about 20%). On the other hand, the presence of sucrose in the medium reduced the chlorophyll content by about 30% and "C-fixation from NaH"4CO3 in the soluble fraction by about 60%. A possible correlation between the influence of kinetin on sugar uptake and the effect of kinetin on "C-fixation from NaH"CO3 was discussed.

During the first 8-12 days of cultivation in a nutrient solution containing IAA, inositol and kinetin freshly isolated carrot root explants develop into a chlorophyllous and photosynthetically active tissue culture. Electron microscopy, low temperature pigment absorption spectra and fluorescence induction profile recording as well as the determination of the activity of some enzymes (ribulosebisphosphate carboxylase, phosphoenolpyruvate carboxylase) and CO2-fixation experiments were carried out. Based on the results, a sequence of developmental stages of the photosynthetic system will be proposed. During the autotropic period from about the 20th day of culture onward the light reaction system of these tissue cultures is quite comparable to that of carrot leaves; however, some differences in the CO2-fixation mechanism were observed.

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