Summary: Troponin T concentration in serum is usually measured by the automated method developed by Boehringer Mannheim for the ES-series of analysers. These instruments need at least 140 μΐ of serum and 700 μΐ of reagents for a single analysis, which takes 90 min. We describe an alternative procedure, using streptavidin-coated microtitre plates, troponin T reagents of Boehringer Mannheim and an ELIS Α-reader to measure the concentration of troponin T. The present assay needs only 30 μΐ of sample and 200 μΐ of reagents, and it takes 75 min; the detection limit is 0.10 μg/l. We also assessed the microtitre plate method for sensitivity and precision and compared the results with those measured by an ES-300 automatic analyser. Both methods have the same measurement range for troponin T of 0.1 to 15 μg/l. For daily routine use of the microtitre plate method we recommend duplicate determinations.