Water used for human consumption must be safe and free from microbes. Storing water in copper has been used since long time in India, also evident in ancient texts of Ayurveda. Present study aims to evaluate the efficacy of various water storing vessels in purifying drinking water especially in terms of antibacterial (anti-Escherichia coli) activity. The water was collected from Waghur River, Jalgaon and stored in different vessels for 5 days like copper, stainless steel and plastic.Bacterial growth was observed before and after storage. We found the significant reduction in the number of coliform bacteria in water stored in copper vessels than stainless steel and plastic vessels. We observed the benefit of using a copper-based device, which is effective and cheap for storage of water and can be used to made bacteria free water.