Present study is an effort to explore the impact of gender and region on the attitude of adolescent girls and boys students towards lesbian and gay people. Present study includes a sample of 100 rural students (50 male and 50 female) and 100 urban students (50 male and 50 female), selected randomly from colleges of Aligarh. Data was gathered by The Attitudes toward Lesbians and Gay Men Scale of Herek.1 Results reveal that adolescents belong to rural areas have more negative attitude toward gay and lesbian people in comparison to urban area students. It was also found that female have more negative attitude towards lesbian and gay people. It can be concluded that students from rural areas are more negative than adolescents from urban areas for Lesbian and Gay. It may be happen because students from urban areas share a combined global culture and more freedom than to the students of rural areas that affects their attitude and thinking. As well as girls face more restrictions than boys that may alter their attitude towards those things that are against laws and culture.