Background: Low phosphorus availability limits crop production in alkaline calcareous soils in semi-arid regions including Pakistan. Phosphate solubilizing bacteria may improve crop growth on alkaline calcareous soils due to their ability to enhance P availability. Methods: Twenty rhizobacterial isolates (Q1-Q20) were isolated from rhizosphere of cotton and characterized for their growth promoting attributes in vitro. The selected phosphate solubilizing isolates were further screened for their ability to improve cotton growth under axenic conditions (jar trial). The phosphorus solubilization capacities of selected strains were quantified and these strains were identified through 16S rDNA sequencing. Results: Isolates Q2, Q3, Q6, Q7, Q8, Q13 and Q14 were able to solubilize phosphate from insoluble sources. Most of these isolates also possessed other traits including catalase activity and ammonia production. The growth promotion assay showed that Q3 was significantly better than most of the other isolates followed by Q6. Maximum root colonization (4.34 × 10 6 cfu g-1) was observed in case of isolate Q6 followed by Q3. The phosphorus solubilization capacities of these strains were quantified, showing a maximum phosphorus solubilization by Q3 (Optical density 2.605 ± 0.06) followed by the Q6 strain. The strain Q3 was identified as Bacillus subtilis (Accession # KX788864) and Q6 as Paenibacillus sp. (Accession # KX788865) through 16S rDNA sequencing. Discussion: The bacterial isolates varied in their abilities for different growth promoting traits. The selected PGPR Bacillus subtilis strain Q3 and Paenibacillus sp. strain Q6 have multifarious growth promoting traits including ability to grow at higher EC and pH levels, and phosphorus solubilizing ability. These strains can efficiently colonize cotton roots under salt affected soils and help plants in phosphorus nutrition. It is concluded that both strains are potential

Background: Low phosphorus availability limits crop production in alkaline calcareous soils in semi-arid regions including Pakistan. Phosphate solubilizing bacteria may improve crop growth on alkaline calcareous soils due to their ability to enhance P availability.Methods: Twenty rhizobacterial isolates (Q1-Q20) were isolated from rhizosphere of cotton and characterized for their growth promoting attributes in vitro. The selected phosphate solubilizing isolates were further screened for their ability to improve cotton growth under axenic conditions (jar trial). The phosphorus solubilization capacities of selected strains were quantified and these strains were identified through 16S rDNA sequencing. Results: Isolates Q2, Q3, Q6, Q7, Q8, Q13 and Q14 were able to solubilize phosphate from insoluble sources. Most of these isolates also possessed other traits including catalase activity and ammonia production. The growth promotion assay showed that Q3 was significantly better than most of the other isolates followed by Q6. Maximum root colonization (4.34 × 10 6 cfu g -1 ) was observed in case of isolate Q6 followed by Q3. The phosphorus solubilization capacities of these strains were quantified, showing a maximum phosphorus solubilization by Q3 (Optical density 2.605 ± 0.06) followed by the Q6 strain.The strain Q3 was identified as Bacillus subtilis (Accession # KX788864) and Q6 as Paenibacillus sp. (Accession # KX788865) through 16S rDNA sequencing. Discussion: The bacterial isolates varied in their abilities for different growth promoting traits. The selected PGPR Bacillus subtilis strain Q3 and Paenibacillus sp. strain Q6 have multifarious growth promoting traits including ability to grow at higher EC and pH levels, and phosphorus solubilizing ability. These strains can efficiently colonize cotton roots under salt affected soils and help plants in phosphorus nutrition. It is concluded that both strains are potential PeerJ reviewing PDF | Manuscript to be reviewed candidates for promoting cotton growth under alkaline conditions, however further investigation is required to determine their potential for field application.PeerJ reviewing PDF | Manuscript to be reviewed 1 Preliminary study on phosphate solubilizing Bacillus subtilis strain Q3 and Paenibacillus 2 sp. strain Q6 for improving cotton growth under alkaline conditions 3 Manuscript to be reviewed 17 Abstract 18 Background: Low phosphorus availability limits crop production in alkaline calcareous soils in 19 semi-arid regions including Pakistan. Phosphate solubilizing bacteria may improve crop growth 20 on alkaline calcareous soils due to their ability to enhance P availability.21 Methods: Twenty rhizobacterial isolates (Q1-Q20) were isolated from rhizosphere of cotton and 22 characterized for their growth promoting attributes in vitro. The selected phosphate solubilizing 23 isolates were further screened for their ability to improve cotton growth under axenic conditions 24 (jar trial). The phosphorus solubilization capacities of selected strains were quantifi...