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For the first time in the Czech Republic, bacteria identified as <I>Pseudomonas marginalis, Pectobacterium carotovorum</I> subsp. <I>carotovorum</I> and <I>Pseudomonas putida</I> were isolated from tubers of <I>Zantedeschia</I> spp. with symptoms of tuber soft rot. The symptoms occurred on mother tubers as well as on new daughter tubers of different calla lily hybrids with yellow spathe, calla lily cv. Mango with bright orange spathe and <I>Zantedeschia rehmanii</I> with pink spathe. The percentage of diseased plants of the total plants in the plot was around 10%. When inoculated into potato tuber slices, strains of <I>P. marginalis</I> and <I>P. c</I>. subsp.<I> carotovorum</I> produced soft rot. Pectolytic activity of <I>P. marginalis</I> strains was less intensive than that of the <I>P. c.</I> subsp. <I>carotovorum</I> strain. The results confirm that bacterial soft rot of <I>Zantedeschia</I> spp. may have several causes.

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Between 2008 and 2010, horse chestnut (Aesculus hippocastanum) trees growing at 216 locations in the Czech Republic were surveyed for bleeding canker disease. Typical symptoms of bleeding canker were found at 16 locations, and samples were collected from five of these locations. The bacterium Pseudomonas syringae was isolated from five locations, and Pseudomonas syringae pathovar aesculi, which is the causal agent of bleeding canker disease, was isolated at one location. This is the first report of P. syringae pv. aesculi in the Czech Republic.