Crossbred beef heifers [N = 1,394; initial shrunk body weight (BW) 291 ± 9.9 kg] were used to investigate the efficacy of 10-G Armor (Life Products, Inc., Norfolk, NE; 10-G) upon feedlot performance, carcass characteristics, and fecal and subiliac lymph nodes Salmonella prevalence. Heifers were blocked by day of arrival and allocated to 1 of 20 pens (N = 70 heifers/pen) and assigned one of two treatments (10 pens/treatment) : no direct fed microbial (CON) or 2g/heifer/d of L. acidophilus, E. faecium, P. pentosaceus, L. brevis and L. plantarum respectively (Life Products, Inc., Norfolk, NE; 10-G). Twenty four animals were randomly selected from each pen for Salmonella sampling. Recto-anal mucosal swab samples (RAMS) were obtained at initial processing and harvest; subiliac lymph nodes were collected at harvest. In addition, pen surface fecal pats were collected and composited by pen (10 pats per composite, 5 composites per pen) on day 0, 52, 120 and 192. Data were analyzed as a generalized complete block design and pen served as the experimental unit. No differences were observed in live growth performance metrics (P ≥ 0.55). Yield grade distributions did not differ between treatments (P ≥ 0.62), however cattle fed 10-G tended (P = 0.06; 14.6 vs 18.9%) to have fewer USDA Select carcasses and more (P = 0.09; 73.6 vs 78.0%) USDA Choice carcasses. Cattle fed 10-G tended (P = 0.10; 9.2% vs 12.3%) to have fewer liver abscesses and had fewer (P = 0.04; 5.3 vs 8.5%) severe liver abscesses. Salmonella prevalence of RAMS did not differ between treatments at initial processing (P = 0.97; CON = 11.6%, 10-G = 11.5%) or at harvest (P = 0.91; CON = 99.0%, 10-G = 98.6%), however RAMS differed (P < 0.01) in Salmonella prevalence between the two collection times. Cattle fed 10-G had a lower frequency of Salmonella positive lymph nodes (P = 0.01; CON = 15.8%, 10-G = 7.4%) than CON. However, Salmonella log (mpn/g) of lymph nodes did not differ between treatments at harvest (P = 0.34; CON = 0.73, 10-G = 0.34). These data indicate that cattle fed 10-G have decreased rates of severe liver abscesses without altering live animal performance or carcass characteristics. Supplementation of 10-G significantly reduced prevalence rate of Salmonella recovered from the subiliac lymph nodes. The factors responsible for the observed difference in the effects of 10-G on Salmonella warrants further investigation