Background: Usage of herbal drugs in traditional medicine is quite well known but largely empirical. Hence the present study was designed to screen the in vitro antifilarial effect of Butea monosperma L. plant leaves against Setaria cervi. Methods: Antifilarial activity of Methanol/Hexane-ethanol extracts of Butea monosperma L. (leaves) and Ciprofloxacin was explored against adult of Setaria cervi after incubation for 24 hrs with concentration range of 0.25 to 20 mg/ml for possible antifilarial effect by comparing with suitable control, in terms of motility inhibition assay and MTT reduction assay. Results: Butea monosperma L. plant leaves showed significant antifillarial activity against adult as compared to controls whereas activity demonstrated by Ciprofloxacin was comparatively less significant. Inhibitory concentrations (IC50) for the plant extracts with significant antifilarial activity against Setaria cervi adult in vitro system have been derived to be 1.25, 3.6 and 7.5 mg/ml Methanol, Hexane-ethanol extracts and Ciprofloxacin respectively. Conclusion: The present research investigation proved to be an additional frame in recording the plant extract's antifilarial activity. Methanol and Hexane-ethanol extracts of Butea monosperma L. plant leaves which shown significant antifilarial activity. The present research data highlights the importance of further depth research in this area.

Background: Staphylococcus aureus has long been recognized as a major pathogen of urinary tract and hospital acquired infections. Over the last decade antibiotic resistance and biofilm formation potential by pathogens is a major challenge. In this study we screened S. aureus clinical isolates by antimicrobial susceptibilty and biofilm assays to determine biofilm capability of vancomycin and ciprofloxacin resistant isolates. Methods: Six clinical isolates of S. aureus were characterized by biochemical tests and further antibiotic susceptibility of vancomycin and ciprofloxacin were tested against S. aureus clinical isolates by disc diffusion method. Biofilm formation capability of these isolates were performed by microtiter plate, coverslip congored agar and tube assays. Results and disscusion:In this study we found that two isolates were resistant to ciprofloxacin and three isolates were resistant to vancomycin, on the basis of CLSI guidelines. Ciprofloxacin resistant isolates showed moderate biofilm formation while vancomycin resistant showed strong biofilm formation. Conclusion:We have concluded that ciprofloxacin and vancomycin resistant clinical isolates were showed differences in biofilm formation. Staphylococcal isolates having biofilm propensity exhibit more resistance to antibiotics, hence are difficult to treat.