This study focuses on knowledge of medicinal plants that are used as raw materials by the sellers of jamu gendong. In addition, this study explores the types of jamu that are produced from those medicinal plants. The qualitative method was applied in this research because it can reveal the phenomenon of informants' knowledge and experiences especially concerning on medicinal plants utilization in producing jamu gendong. The research was conducted in Wonolopo Village, Mijen, Semarang, in 2018. There were 20 jamu gendong sellers volunteered in this study. There are 26 species of medicinal plants and 16 plant families are usually used as raw material in making jamu gendong.