The influence of systematic application of mineral fertilizers, on the background (BG) of the after-effect of 30 t on ha of manure, on the content of nutrients in spring wheat plants of “Myronivska Yara” regional variety and winter wheat plants of “Myronivska 61” variety, and on nutrients removal by plants, during cultivation on Meadow-Chernozemic Carbonate Soil, was studied. The results indicate a close relationship between external conditions and internal metabolic processes in winter and spring wheat plants. Thus, a balanced ratio of nitrogen, phosphorus and potassium provides a more intensive supply of these elements from the soil, what increases their content in plants. The highest content of total nitrogen, phosphorus and potassium in winter wheat plants was observed in the variant, where 1.5 rate of mineral fertilizers was applied with the background of 30 t on ha of manure, in the tillering phase and was 4.86; 2.17; 3.48 %, respectively, what is almost 2 times higher, than the control. During the growing season, the nutrients content in all variants decreased. The same pattern was observed in plants of spring wheat. The maximum nutrients content was recorded in all variants in the phase of spring tillering, and also was recorded significant reduction in their adsorption from the soil and using by plants during the growing season. The content of total nitrogen in spring wheat plants in the tillering phase fluctuated in fertilized variants in the range of 2.21-2.51 %, compared with the control – 2.10 %; phosphorus – in the range of 1.20-1.47 %, compared with the content on the control – 1.04 %; potassium – 1.88-3.08 %, in the control, respectively – 1.68 %. The results of research indicate, that long-term application of fertilizers in crop rotation affects, as a result, also on the removal of nutrients with the crop. Thus, the removal of basic nutrients by plants of winter and spring wheat was the highest in the variant “BG + N110P120K120”. Under winter wheat it was 210 kg on ha N, 122 P2O5, 157 kg on ha K2O, compared with the control variant – 86.3 N, 48.2 P2O5, 57.8 kg on ha K2O. Under the spring wheat it was 119 N, 66.4 P2O5, 85.4 kg on ha K2O, and in control variant, respectively, – 46.6 N, 26.5 P2O5, 32.8 kg on ha K2O.