Background and Aims: Some studies bring different visions of innovation among the stakeholders of the wine sector; however, there is a gap in our understanding of the characteristics of and the relationship between the ecosystem and the stakeholders. This study aims to identify and measure critical success factors (CSFs) of the wine innovation ecosystem. Methods and Results: A mixed-method approach with 29 qualitative questionnaires for wine managers, and a survey of 138 wine managers from Brazilian wine regions were employed. The survey validated 30 critical factors that were identified from the literature and which were validated in the qualitative research. The critical factors were then submitted to a support factorial analysis, resulting in 27 CSFs related to six new dimensions of the wine ecosystem. The dimensions identified by the factorial analysis were evaluated by indicators and measured for four clusters of companies. Conclusions: The study identified the dimensions and CSFs related to four Brazilian winery clusters: (i) medium businessbusinesses focused on social and economic entrepreneurship; (ii) large business-focused on entrepreneurship and leadership in the development of sustainable products and services; (iii) startup-expanding businesses focused on economic growth; and (iv) small business-focused on entrepreneurship and innovation for a sustainable economy. Significance of the Study: This study demonstrated the benefit of using dimensions and CSFs to create innovative strategies to improve the competitiveness and performance of the wine ecosystem.