Medicinal plants based drugs and chemicals used for curing enormous ailments and it help for the invention of modern pharmaceuticals. The target of this present study is to find out the bioactive compounds and to evaluate antiproliferative and larvicidal effect on Gliricidia sepium leaves. Phytochemical determination was oriented to search secondary metabolites and this preliminary study shows Gliricidia sepium it has alkaloid, flavanoids, phytosteroids, steroids, tannins, terpenoids, mucilage, and coumarins. In this Research a significant cytotoxic effect was observed in Gliricidia sepium methanolic extract against Adenocarcinoma gastric cells. The larvicidal activity was observed by using aqueous and methanolic plant extracts on Anopheles mosquitoes and shows a high mortality rate on 48 hours after the plant extracts exposure. The result of this study shows this plant has significant phytochemicals and potential antiproliferative and Larvicidal effects.