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Nellore Jodipi cyclic ewes were synchronized to assess the effectiveness of two different doses of double PGF2á (Cloprostenol sodium) @ either 125 or 250 µg with 9 d interval in Groups I and II based on the estrus response and fertility rate after intra cervical inseminations at 60 and 72 h after 2nd PGF2a. The estrus response (%), onset of estrus (%), duration of estrus (h) and intensity of estrus in ewes synchronized by PGF2a were 80.95 and 85.71; 50.94+3.12 and 48.00+3.40; 24.35+1.41 and 28.22+1.15 and 6.76+0.29 and 6.83+0.34 in Groups I and II, respectively. The conception rate based on 25 d non return rate was 85.71 and 80.95 % with an overall lambing rate of 71.43 and 66.67 % in Group I and II, respectively. The study revealed that the synchronization response among the treated ewes by 125 µg of PGF2á was slightly lesser with higher lambing rate than 250 µg of PGF2a.