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Shoot cultures were initiated from seedling shoot tips of Brassica campestris ssp. pekinensis (Lour.) Olsson (Chinese cabbage, cv. Kimjung) on Murashige–Skoog medium supplemented with 46.5 μm kinetin and 1.5 μm IBA. Proliferation was a result of growth from axillary buds. Adventitious shoots could not be induced on a wide range of culture media. The effects of cytokinin and auxin type and concentration, nitrate : ammonium ratio, sucrose concentration, and other additives on shoot multiplication and growth were determined. Only adenine sulfate was beneficial. Expiants were rooted on hormone-free medium, but addition of 2.5 or 5.0 μm IAA or IBA increased rooting and enhanced shoot growth. Regenerated plants were morphologically normal and exhibited flowering during both root initiation in vitro and after establishment in soil. Chemical names used: N-(2-furanylmethyl)-1H-purin-6-amine (kinetin); 1H-indole-3-butanoic acid (IBA); 1H-indole-3-acetic acid (IAA).