Aim: Pelvic organ prolapse (POP), although not life-threatening, is a serious condition that can affect a patient's quality of life. As life expectancy rises, the number of women with POP continues to rise. The weakness of the pelvic floor is always found underlying each case of POP. This weakness in the pelvic floor is associated with repeated damage to muscles and connective tissue due to the process during pregnancy and delivery. Balinese women have risk factors for developing POP related to the obligation to continue their offspring by conceiving and giving birth more than 4 times and working as heavy workers. The presence of genetic factors is also thought to play a role as a risk factor for POP in Balinese women. The purpose of this study was to prove the existence of polymorphism matrix metalloproteinase-9 (MMP-9) rs17576 as a factor that puts Balinese women at risk for POP. Materials and methods: A paired case-control study was conducted on each of 30 Balinese women with POP and without prolapse, which was done matching based on parity and occupation variables at