In this study, multi-elemental analysis and antioxidant activity of samples belonging to different locations of Helichrysum arenarium (L.) Moench known as the immortal flower was determined. The results obtained were compared within themselves. The Ca, Mg, Al, Fe, Na, Li, Be, B, Ti, Cr, Ni, Zn, Mo, and Pb element contents of the sample belonging to the B location were determined that were higher than that of the other location. The K, V, Mn, Co, Cu, As, Se, Sn, and Ba element contents of the sample belonging to the G location were determined that were higher than that of the other location. When the antioxidant activity results of the species were evaluated; according to the CUPRAC (Cupric Reducing Antioxidant Capacity) method, the sample extracts of the B and G locations were determined that showed lower activity than the standard BHA, BHT, and Trolox values at 20 and 40 µg/mL concentrations, and showed close activity compared to the standards at 80 µg/mL concentrations. The species was determined that be antioxidant activity even at low concentrations. According to the DPPH (2,2-difenil-1-pikrilhidrazil) method, the antioxidant activity of the extract of the B and G locations was determined as 22.95 and 23.76 mg TE/mL, respectively. According to the results of the bivariate analysis of the species; considering the p < 0.01 and 0.05 confidence level of the sample belonging to the B location, it was determined that the strongest correlation was between Na and Al (r = -0.888), and the strongest correlation of the sample belonging to the G location was between Al and B (r = 0.844).
