The diet of the Short-eared Owl Asio flammeus was analysed in an arid environment in Hassi El Gara located in the southeast of El Golea (Ghardaia, Algeria). The diet was determined by analysing 138 pellets. Our data showed that the diet was dominated by mammals (Chiroptera and Rodentia). Based on relative biomass, birds were the main prey species. Mammals were the second most important prey. Mammals were the major food item throughout the seasons and their contribution to the diet ranged from 50.7% in spring to 73.6% in summer. Birds were the second numerous prey with 8.1% in summer and 29.6% in spring. The dominant prey species was Myotis sp., making up 37.8%. It was followed by Gerbillus nanus (5.4%), Columba livia (4.3%) and Bufo mauritanicus (4.1%).