The results of present investigation revealed that among the different potting media treatments, M1: Vermiculite + Garden soil + Sand was found to be most beneficial for improving took days to germination, germination percentage, height of the seedling, number of leaves seedling-1, stem diameter, primary roots length (cm), number of secondary roots, fresh and dry shoots weight, fresh and dry roots weight, seed vigor index – I and II and leaf area (cm). In present investigation, application of G3: GA3 600 ppm significantly enhanced the seed germination and seedling growth viz., Days to germination (25.12), germination percentage (91.52%), height of the seedling (32.78cm), number of leaves seedling-1(18.61), stem diameter (3.53), primary roots length (7.59cm), number of secondary roots (21.68), fresh and dry shoots weight (16.71g and 6.51g), fresh and dry roots weight (1.93g and 1.31g), seed vigour index – I (3000.11) and II (699.87) and leaf area (66.33 cm2). The effect of interaction of different level of GA3 (0.00, 400, 500 and 600ppm) and different level of potting media viz., vermicuilite, garden soil, sand, FYM, cocopeat and vermincompost (1:1:1 ratio) on seed vigour index - I. The maximum seed germination, days to germination, and seedling growth, seed vigour index I & II and leaf area was found in treatment GA3 600 PPM + Vermiculite + Garden soil + Sand. The effect of interaction of GA3 and potting media was found significant for seed germination, days to germination, and seedling growth, seed vigour index I & II and leaf area of guava cv. Allahabad safeda seedling under shade net condition.