An experimental study of microstrip patch antenna designed and fabricated on FR4 epoxy substrate is presented. Further a performance comparison of designed antenna is made with proposed design using Gallium doped Ba-Sr hexagonal ferrite substrate. Microstrip feed line is used for inputting the signal to antenna. The whole simulation is done on HFSS simulator (version 13.0).The center frequency for proposed antenna is 10GHz and is optimized for significant performance parameters viz return loss, bandwidth, VSWR and gain. It was observed that the designed antenna provides better results with ferrite substrate as compared to FR4 epoxy substrate showing -10db broad bandwidth of 4.2GHz in the frequency region 8.2GHz to 12.4GHz. Although, the results of other parameters like return loss, VSWR and gain are found to be optimum with FR4 substrate as compared to mentioned ferrite substrate. The prototype of proposed antenna with FR4 epoxy substrate is fabricated and tested to attain the experimental results. The measured results are found to be better than simulated results. Thus the proposed antenna structure can be considered suitable for microwave communication application in X-band.