New unsymmetrical Schiff bases containing azomethine moiety with simple aromatic section in one side and ferrocene fragment attached imine on the other side have beensynthesized. Advent of metal-to-ligand charge transfer band for the coordination of Cu2+ ions with receptors and appropriate changes in UV-Visible spectra for other metal ion combination with the sensor is reported. Observed extravagant ΔEpvalues suggest quasi-reversible process. The ΔIpa amount calculated from the anodic current Ipa value noticed for receptor solution and different metal ion added sensor solution discloses the concentration of metal ions required for effective sensing. The synthesized ligands were subjected to antimicrobial activity against four bacterial and two fungal stains and the zone of inhibition (in mm) was calculated. Further molecular docking study was carried out and the binding energy (Kcal.mol-1) for the synthesized ligand (R1 and R2) with the selected protein was intended.