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Nanocrystalline film of Zinc Oxide (ZnO) nanowires were prepared on glass substrate at a low temperature using a low cost, simple hydrothermal method. We used different concentration of ZnO nanowires precursor solution and studied the effect on its structural, morphological and optical properties. The morphology and structural properties of the ZnO nanowires were studied using scanning electron microscopy (SEM), and x-ray diffraction (XRD) respectively. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) results confirm that the nanorods were single crystals with the wurtzite structure. Optical transmittance (UV-Vis) and photoluminescence (PL) properties of ZnO nanorods have been characterized at room temperature.