Amisulbrom is a novel quinone inside inhibitor, which
exhibits
excellent inhibitory activity against phytopathogenic oomycetes. However,
the resistance risk and mechanism of amisulbrom in Phytophthora litchii are rarely reported. In this
study, the sensitivity of 147 P. litchii isolates to amisulbrom was determined, with an average EC50 of 0.24 ± 0.11 μg/mL. The fitness of resistant mutants,
obtained by fungicide adaption, was significantly lower than that
of the parental isolates in vitro. Cross-resistance
was detected between amisulbrom and cyazofamid. Amisulbrom could not
inhibit the cytochrome bc1 complex activity with H15Y and G30E + F220L
point mutations in cytochrome b (Cyt b) in vitro.
Molecular docking indicated that the H15Y or G30E point mutation can
decrease the binding energy between amisulbrom and P. litchii Cyt b. In conclusion, P.
litchii might have a medium resistance risk to amisulbrom,
and a novel point mutation H15Y or G30E in Cyt b could cause high
amisulbrom resistance in P. litchii.
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