Objective: This study is conducted to ascertain the microbial agents along with their prevalence leading to urinary tract infections in patients admitted in Bakhtawar Amin Hospital, Multan with manifestations of urinary tract infections. Design: Descriptive observational study Place and duration of study: Department of Microbiology Bakhtawar Amin Medical & Dental College & Hospital, Multan from January 2021 to July 2021. Material and Methods: A total of 220 positive urine cultures were analyzed in this study extended for six months. All the appropriate details like age, gender, major presenting complaints, history of catheterization etc were noted. All the isolated urinary pathogens were recorded along with their frequency and percentages. Results: Out of these 220 patients with positive urine cultures, majority (63.6%) were male. Gram negative bacteria (E. Coli) constitute the major bulk of urinary pathogens followed by Candida. Conclusion: Urinary tract infections were commonly seen in men in our region. E. Coli was the most frequently encountered bacteria. A large number of Candida species were segregated in immunocompromised critically ill ICU admitted patients. Keywords: Urinary tract infection, culture, pathogens, E.Coli, Candida.