Background: Many factors influence the composition of saliva. The rate of secretion follows a circadian rhythm, decreasing during sleep and increasing during day. The present study was conducted to assess pH level and uric acid in saliva before and after complete denture insertion. Materials & Methods: 80 completely edentulous patients of both genders were divided into two groups of 40 each and each group was further divided into two groups of 20 each. Group I was age ranged 40-60 years contains two subgroups: group A1 for smokers and group A2 for non-smokers. Group II age ranged 61-80 years contains two subgroups: group B1 for smokers and group B2 for non-smokers. Saliva samples were collected from subjects immediately before the insertion of dentures and pH & uric acid estimation was done. Results: Out of 80 patients, males were 45 and females were 35. The mean pH of saliva before denture insertion was 6.7 and after denture insertion was 5.1. The difference was significant (P< 0.05). The mean uric of saliva before denture insertion was 4.5 and after denture insertion was 3.7. The difference was significant (P< 0.05). There was significant difference in pH and uric acid level while comparing A1 vs A2 and B1 vs B2 (P< 0.05). Conclusion:There was no significant decrease in levels of salivary pH and uric acid in complete denture wearer.