The current study aimed to detect human cytomegalovirus among infertile individual. Blood and seminal fluid collected of 300 infertile male randomly, ELISA technique was used to know the activity of humoral immunity among them through measuring anti-HCMV IgM and IgG antibodies, the result revealed that 31 (11%) were positive for IgM result and 234 (78%) were positive for IgG. The PCR technique showed that out of 300 samples only 22 (7.4%) gave positive results, In case of control group, results were negative for anti- HCMV IgM antibodies in ELISA test and PCR technique. While IgG gave a weak positive results. Objective: the present study was to study the relationship between the virus and infertility through detection of human cytomegalovirus (HCMV) infection in infertile male in AL-Najaf governorate by screening of anti-human cytomegalovirus IgM and IgG in the serum and detection of human cytomegalovirus DNA in seminal fluid, using PCR technique.

IL-2 plays a pivotal role in the induction of host immune responses against tumor growth and it has anti-tumor activity. Therefore the present study aimed at shedding light on a group of Iraqi patients with multiple myeloma by evaluating the level of this cytokine in their sera . The age and gender were also taken into consedration. and investigate if there was any association between IL-2 and the level of β2-m. A group of 49 patients and 22 healthy volunteers were included in the study. The quantitative determination of IL-2 serum level was performed by ELISA. The patients were divided into two groups: the first group included (17) patients who were recently diagnosed and not received any treatment at the time of collecting samples, while the second group included (32) patients who received treatment. Results: A significant decrease in the serum level of IL-2 was observed in MM patients (3.89 ± 3.64 vs. 11.61 ± 23.37 U/ml) as compared with controls. There were no significant difference in level of IL-2 between the patients who received treatment and those who did not as well as there was no association between production of IL-2 one hand and production of β2-m and IgG on the other hand . Finally there was no association between age and IL-2. The serum level of this interleukin showed no gender-related variations between males and females and there was no correlation between it (IL-2) and gender factor.