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Glyceline, reline, or ethaline deep eutectic solvents and carbon black nanoparticles within a crosslinked chitosan film are investigated as glassy carbon electrode modifiers for the first time. The selected 5 mg mL−1 glyceline modified GCE was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy. Simultaneous determination of acetaminophen and diclofenac by differential pulse adsorptive stripping voltammetry (DPAdSV) presented limits of detection of 2.6×10−8 and 5.2×10−8 mol L−1 for acetaminophen and diclofenac, respectively, in pharmaceutical and biological samples. The obtained results were compared with those obtained by HPLC at a confidence level of 95 %.

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