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Extracts of germinated barley contain two debranching enzymes which have been separated from each other, and from α‐amylase, by continuous electrophoresis followed by gel filtration. Amylopectin 6‐glucanohydrolase hydrolyses the outer‐most inter‐chain linkages in amylopectin but has no action on glycogen or on α‐limit dextrins. Limit dextrinase has no action on amylopectin, but readily hydrolyses α‐limit dextrins; it differs fron amylopectin 6‐glucanohydrolase in having a relatively lower activity at pH 4, pH 7 or 60° C, and is selectively activated by chloride ions.

35P ofits conversion into fibrous hydroglucan (Houwink & Kreger, 1953). We thank Dr C. T. Bishop for a sample of glucan from Candida parap8ilo8i8 and Mrs E. F. Cruickshank for technical assistance.

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