Antifouling coatings were prepared with paints containing polyaniline (PAni) and derivatives as active pigment, and evaluated by antifouling performance on metallic or polyvinyl chloride substrates. The paints, PAni and its derivatives were characterized by FT-IR spectrophotometry, thermogravimetric analysis, electrical conductivity and scanning electron microscopy. Coatings were also characterized by salt spray, leaching and erosion tests. Antifouling coatings' performance was evaluated by immersion tests in a marine environment or in fresh water. Paints containing PAni-ES, PAni/DBSA and SPAN, and a co-biocide PyZn, showed antifouling performance similar to a commercial antifouling paint.