These observations suggest that collagen hydroxylation of matrix by vitamin C does not play a role in this model of chondrocyte-dependent collagen degradation. Also, this study demonstrates that chondrocyte-derived lipid peroxidation product MDA mediates oxidation of cartilage collagens. Oxidative modification of cartilage collagen in vivo could result in alteration of biochemical and biophysical properties of cartilage collagen fibrils, making them prone to degradation, thus initiating the changes observed in aging and OA.

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Common mental health problems seen among the college students are depression, suicide, anxiety disorder, eating disorder, substance use etc. which may rise in young adulthood. This study was done to assess depression anxiety stress (DAS) among the college students in selected area of Goa. The objectives of the study were a) To assess Depression Anxiety stress (DAS) among the college students based on Depression Anxiety Stress Scale – 42 (DASS- 42). b) To associate the level of Depression, Anxiety and Stress (DASS) score with selected demographic variables of the college students. A quantitative approach was used with descriptive research design. Convenient sampling technique was used. Sample size was 85. The investigator used Depression Anxiety Stress Scale (DASS)-42 tool to assess Depression Anxiety Stress (D, A, S) among students. Descriptive and inferential statistics were used to analyze the data. The results revealed that 47(55.3%) had normal level of Depression, 20 (23.5%) had moderate level of Depression yet 2 (2.4%) suffered from severe depression, Majority of the subjects 24 (28.2%) has moderate Anxiety, 15 (17.6%) has severe Anxiety and 6 (7%) has very severe Anxiety. Majority of the subjects 12 (14.1%) has moderate Stress, 2 (2.4%) had severe and none had very severe Stress. The finding of the study showed varied levels of depression, anxiety and stress among the college students. So, screening and creating more awareness and intervention strategies may be needed for the better mental health of the students.

Flurbiprofen (FLB) and methyl salicylate (MS), the actives in a commercial topical gel formulation were extracted for their simultaneous estimation. The extraction system was optimized, validated for elimination of interferences associated with matrix materials used in the formulation and also compensate for possible interference of one drug by the other during analysis. An efficient extraction procedure was developed for the separation of flurbiprofen and methyl salicylate from the marketed formulation Brugel and analyzed for the content with a new validated vierodt's simultaneous equation method. Detection wavelengths of 248 and 304 nm were used for recording the absorbance as they represent the absorption maxima of flurbiprofen and methyl salicylate respectively. Simultaneous equations were designed to estimate the unknown concentration of both the components in the marketed formulation. Linearity was calculated using standard stock solution of methyl salicylate and flurbiprofen in the concentration range of 5-30 µg/mL. The developed method was found to be specific, precise, accurate and can be used for routine analysis of flurbiprofen and methyl salicylate in marketed gel formulations.