Resumen: Urbanization growth in developing countries pushes the implementation of environmentally friendly modes of urban transportation. Non-motorized mobility, particularly bike sharing system (BSS) has become an important mode of transportation in many cities worldwide. However, in Latin America, the potential impact of BSS has been barely explored. This study use System Dynamics (SD) to evaluate the performance of a BSS integrated into the public transportation network of a large Mexican city. Several scenarios are simulated by changing critical external factors that comprise the five subsystems of an SD model. The statistical analysis of the simulation results identifies the variables that significantly affect the long-term performance of the BSS in terms of the number of cycling journeys and the reduction of carbon dioxide emissions. Recommendations regarding how to improve the BSS infrastructure and promote its use are suggested after these findings.