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SummarySoya bean (Glycine max (L.) Merr.) is sought after for both its oil and protein components. Genetic approaches to add value to either component are ongoing efforts in soya bean breeding and molecular biology programmes. The former is the primary vegetable oil consumed in the world. Hence, its primary usage is in direct human consumption. As a means to increase its utility in feed applications, thereby expanding the market of soya bean coproducts, we investigated the simultaneous displacement of marine ingredients in aquafeeds with soya bean‐based protein and a high Omega‐3 fatty acid soya bean oil, enriched with alpha‐linolenic and stearidonic acids, in both steelhead trout (Oncorhynchus mykiss) and Kampachi (Seriola rivoliana). Communicated herein are aquafeed formulations with major reduction in marine ingredients that translates to more total Omega‐3 fatty acids in harvested flesh. Building off of these findings, subsequent efforts were directed towards a genetic strategy that would translate to a prototype design of an optimal identity‐preserved soya bean‐based feedstock for aquaculture, whereby a multigene stack approach for the targeted synthesis of two value‐added output traits, eicosapentaenoic acid and the ketocarotenoid, astaxanthin, were introduced into the crop. To this end, the systematic introduction of seven transgenic cassettes into soya bean, and the molecular and phenotypic evaluation of the derived novel events are described.

Effective forest management, particularly during forest disturbance events, requires timely and accurate monitoring information at appropriate spatial scales. In Hawai‘i, widespread ‘ōhi‘a (Metrosideros polymorpha Gaud.) mortality associated with introduced fungal pathogens affects forest stands across the archipelago, further impacting native ecosystems already under threat from invasive species. Here, we share results from an integrated monitoring program based on high resolution (<5 cm) aerial imagery, field sampling, and confirmatory laboratory testing to detect and monitor ‘ōhi‘a mortality at the individual tree level across four representative sites on Hawai‘i island. We developed a custom imaging system for helicopter operations to map thousands of hectares (ha) per flight, a more useful scale than the ten to hundreds of ha typically covered using small, unoccupied aerial systems. Based on collected imagery, we developed a rating system of canopy condition to identify ‘ōhi‘a trees suspected of infection by the fungal pathogens responsible for rapid ‘ōhi‘a death (ROD); we used this system to quickly generate and share suspect tree candidate locations with partner agencies to rapidly detect new mortality outbreaks and prioritize field sampling efforts. In three of the four sites, 98% of laboratory samples collected from suspect trees assigned a high confidence rating (n = 50) and 89% of those assigned a medium confidence rating (n = 117) returned positive detections for the fungal pathogens responsible for ROD. The fourth site, which has a history of unexplained ‘ōhi‘a mortality, exhibited much lower positive detection rates: only 6% of sampled trees assigned a high confidence rating (n = 16) and 0% of the sampled suspect trees assigned a medium confidence rating (n = 20) were found to be positive for the pathogen. The disparity in positive detection rates across study sites illustrates challenges to definitively determine the cause of ‘ōhi‘a mortality from aerial imagery alone. Spatial patterns of ROD-associated ‘ōhi‘a mortality were strongly affected by ungulate presence or absence as measured by the density of suspected ROD trees in fenced (i.e., ungulate-free) and unfenced (i.e., ungulate present) areas. Suspected ROD tree densities in neighboring areas containing ungulates were two to 69 times greater than those found in ungulate-free zones. In one study site, a fence line breach occurred during the study period, and feral ungulates entered an area that was previously ungulate-free. Following the breach, suspect ROD tree densities in this area rose from 0.02 to 2.78 suspect trees/ha, highlighting the need for ungulate control to protect ‘ōhi‘a stands from Ceratocystis-induced mortality and repeat monitoring to detect forest changes and resource threats.