The antibacterial activity of the crude root extracts of the medicinal plant species, Hypochaeris radicata was studied against 15 human pathogen bacteria which includes both Gram-positive and Gramnegative strains by using agar well diffusion method. The chloroform and ethyl acetate extracts displayed broad spectrum activity against all the tested organisms but water extract showed no activity. The petroleum ether and methanol extracts showed only moderate activity. The antibacterial activity of the extracts was compared to the standard Ampicillin. The results of this present study support the plant have good antibacterial potential.