We have studied store-operated Ca 2ϩ entry (SOCE) in Bergmann glia and granule cell layer astrocytes in acute brain slices of the rat cerebellum, using the Ca 2ϩ -sensitive fluorescent dye Fluo-4 and confocal laser scanning microscopy. Astrocytes were identified by their morphology, location, and their Ca 2ϩ response in K
2؉ rather than a microdomain exclusive to CRAC channels is the trigger. Hence a subplasmalemmal Ca 2؉ increase in mast cells is highly versatile in that it triggers cytoplasmic responses through generation of intracellular messengers as well as long distance changes through increased secretion of paracrine signals.
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