The success of endodontic treatment not only lies in meticulous cleaning, perfect shaping and precise filling of canels, it also embodies vital importance on familiarity of unusual root canal patterns. This article presents a case report of a maxillary first molar that had two separate palatal roots with distinct canals and also an invitro assessment of an extracted tooth which had a a similar root canal morphology using cone beam computed tomography (CBCT). Both of them highlight the importance of knowing the anatomical variations to achieve a functional and biological favourable outcome in endodontic treatment.