Ala and Ala-Ma-Ala-Pro, were synthesized and measured in DzO by 13Cn.m.r. spectroscopy. The spectra analysis led us to the conclusion that for each model (irrespective of pD) in conformational equilibrium, the predominant conformation is the one in which side methyl of alanine preceding proline residue eclipses alanine carbonyl group. The influence of pD changes on cis-trans isomerism of Ala-Pro amide bond was also investigated.
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